Training materials

Publications

doi: 10.1007/s43152-020-0001-0 Brown G., Kutner A. and Marcinkowska E. (2020) Vitamin D and hematopoiesis. Current Tissue Microenvironment Reports.
doi: 10.3389/fcell.2020.00012 Brown G, Vicente-Dueñas C and Sánchez-García I. (2020) Editorial: Epigenetic Reprogramming and Cancer Development. Frontiers in Cell and Developmental Biology, 8, 12.
doi: 10.3389/fcell.2019.00104 Brown G and Ceredig R. (2019) Modelling the hematopoietic landscape. Frontiers in Cell and Developmental Biology. 7, 104, 1-13.
doi: 0.1084/jem.20181444 Klein F., Mitrovic M., Roux J., Engdahl C., von Muenchow L., Alberti-Servera L., Fehling H. J., Pelczar P., Rolink A. and Tsapogas P. (2019) The transcription factor Duxbl mediates elimination of pre-T cells that fail β-selection Journal of Experimental Medicine. 216, 638-655.
doi: 10.3389/fimmu.2018.02258 Klein F., von Muenchow L., Capoferri G., Heiler S., Alberti-Servera L., Rolink H., Engdahl C, Rolink M., Mitrovic M, Cvijetic G., Andersson J., Ceredig R., Tsapogas P. and Rolink A. (2018) Accumulation of multi-potent haematopoietic progenitors in peripheral lymphoid organs of mice over-expressing Interleukin-7 and Flt3-ligand. Frontiers in Immunology. 9: 2258.
doi: 10.3390/ijms19072122 Brown G., Ceredig, R. and Tsapogas P. (2018) The making of hematopoiesis: developmental ancestry and environmental nurture. International Journal of Molecular Sciences. 19: 2122.
doi: 10.1021/acs.cgd.8b00091 Wanat M., Malinska M., Kutner A and Wozniak K. (2018). Effect of vitamin D conformation on interactions and packing in the crystal lattice. Crystal Growth and Design 3385. 339
doi.org/10.3389/fimmu.2018.01312 Calvo-Asensio I., Sugrue, T., Bosco N., Rolink A and Ceredig R. (2018). DN2 thymocytes activate a specific robust DNA damage response to ionizing radiation-induced DNA double-strand breaks. Frontiers in Immunology. 9: 1312.
doi: 10.3389/fphys.2018.00439 Calvo-Asensio I., Dillon, E., Lowndes N. F. and Ceredig R. (2018). The role of Hif-1 in enhancing the radioresistance of mouse MSCs. Frontiers in Physiology. In press. 9.439.
Tsapogas P., Brown G., and Ceredig R. (2018). The role of interleukin-7 during haematopoiesis. Chapter 2 In “Advances in Medicine and Biology Volume 131" ed. LV Berhardt. Nova Science Publishers, Inc., New York. pp 101-150.
doi: 10.1111/imcb.12055 Brown G., Tsapogas P., and Ceredig R. (2018). The changing face of haematopoiesis. A spectrum of options is available to stem cells. Immunology and Cell Biology. In press.
doi: 10.3390/ijms18122577 Brown G. and Marcinkowska E (2017). Editorial: Acute myeloid leukaemia: New targets and therapies. International Journal of Molecular Sciences. 18: 2577.
doi: 10.3390/ijms18102162 Szybinski M., Brzeminski P., Fabisiak A., Berkowska K., Marcinkowska E., and Sicinski R. R. (2017). Seco-B-Ring Steroidal Dienynes with Aromatic D Ring: Design, Synthesis and Biological Evaluation. International Journal of Molecular Sciences, 18: 2162
doi: 10.15252/embj.201646638 Alberti-Servera L., von Muenchow L., Tsapogas P., Capoferri G., Eschbach K., Beisel C., Ceredig R., Ivanek R., and Rolink A. (2017). Single-cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors. The EMBO Journal, 36: 3619-3633.
doi: 10.3390/ijms18071492 Watts, J. M, Perez, A., Pereira, L., Fan, Y-S., Brown, G., Vega, F., Petrie, K., Swords, R. T., and Zelent A. (2017) A case of AML characterized by a novel t(4;15)(q31;q22) translocation that confers a growth-stimulatory response to retinoid-based therapy. International Journal of Molecular Sciences, 18: 1492.
doi: 10.3390/ijms18061115 Tsapogas, P., Mooney, C. J., Brown, G. and Rolink, A. (2017) The cytokine Flt3-ligand in normal and malignant hematopoiesis. International Journal of Molecular Sciences. 18: 1115
doi: 10.3390/ijms18051037 Mooney, C. J., Cunningham, A., Tsapogas, P., Toellner K-M. and Brown, G. (2017) Selective expression of Flt3 within the mouse hematopoietic stem cell compartment: Implication for acute myeloid leukaemia. International Journal of Molecular Sciences. 18: 1115
doi: 10.3389/fimmu.2017.00418 Calvo-Asensio, I., Barthlott, T., von Muenchow, L., Lowndes, N.F., Ceredig, R. (2017) Differential response of mouse thymic epithelial cell types to ionizing radiation-induced DNA damage. Frontiers in Immunology. 8: 418

doi: 10.1002/eji.201646638 von Muenchow, L., Tsapogas, P., Albertí-Servera,l., Capoferri,G., Doelz,M., Rolink, H., Bosco, N., Ceredig, R., and Rolink, A. (2016) Pro-B cells propagated in stromal cell-free cultures reconstitute functional B-cell compartments in immunodeficient mice. European Journal of Immunology.47: 394-405.

doi: 10.1073/pnas.1613316113 von Muenchow, L., Alberti-Servera, L., Klein, F., Capoferri, G., Finke, D., Ceredig, R,. Rolink, A. and Tsapogas, P. (2016) Permissive roles of cytokines interleukin-7 and Flt3 ligand in mouse B-cell lineage commitment. Proceedings of the National Academy of Science USA. 113: E8122-E8130.

Brown, G., Kutner, A. and Marcinkowska, E. (2016) Chapter 6: Vitamin D and leukaemia. In “Extraskeletal Effects of Vitamin D”. Ed: EP Liao. Pub: Springer, New York. ISBN 978-3-319-73742-3.

Zhang, Y., Garcia-Ibanez, L., Brown, G. and Toellner, K-M (2016). Detecting gene expression in lymphoid microenvironments by laser microdissection and quantitative RT-PCR. In “Germinal Centers: Methods and Protocols”. Ed: D Caldo. Pub: Springer, New York. 1623, pp 21-36.

doi: 10.1007/s00005-016-0411-0 Brown, G., Marchwicka, A., Cunningham, A., Toellner, K-M. and Marcinkowska, E. (2017) Antagonizing retinoic acid receptors increases myeloid cell production by cultured human hematopoietic stem cells. Archivum Immunologiae et Therapiae Experimentalis. 65: 69-81.

doi: 10.1016/j.imlet.2016.07.010 Ribeirio, A., Ritter, T., Griffin, M. and Ceredig, R. (2016) Development of a flow cytometry-based potency assay for measuring the in vitro immunomodulatory properties of Mesenchymal Stromal Cells. Immunology Letters. 177: 38–46.

doi: 10.1097/MIB.0000000000000964 Slevin, S. M., Dennedy, M. C., Connaughton, E., Ribeiro, A., Ceredig, R., Griffin, M. D. and Egan, L. (2016) Infliximab Selectively Modulates the Circulating Blood Monocyte Repertoire in Crohn's Disease. Inflammatory Bowel Diseases. 22(12):2863-2878.

doi: 10.3390/ijms17071068 Nachliely, M., Sharony, E., Bolla, N. R., Kutner, A. and Danilenko, M. (2016) Prodifferentiation activity of novel vitamin D2 analogs PRI-1916 and PRI-1917 and their combinations with a plant polyphenol in acute myeloid leukemia cells. International Journal of Molecular Sciences. 17: 1068.

doi: 10.1080/13543776.2016.1205586 Marchwicka, A., Cunningham, A., Marcinkowska, E. and Brown, G. (2016) Therapeutic use of selective synthetic ligands for retinoic acid receptors: a patent review. Expert Opinion on Therapeutic Patents. 26, 957-971.

doi: 10.1016/j.intimp.2016.05.009 Gill, S. K, Islam, N., Shaw, I., Ribeiro, A., Bradley, B., Brien, T., Kilcoynea, M., Ceredig, R. and Joshi, L. (2016) Immunomodulatory effects of natural polysaccharides assessed in human whole blood culture and THP-1 cells show greater sensitivity of whole blood culture. International Immunopharmacology. 36: 315-323.

doi: 10.3390/ijms17050729 Marcinkowska, E., Wallace, G. R. and Brown, G. (2016) The use of 1α,25-dihydroxyvitamin D3 as an anticancer agent. International Journal of Molecular Sciences. 17: 729.

doi: 10.1111/imr.12396 Zhang, Y., Garcia-Ibanez, L. and Toellner, K-M. (2016) Regulation of germinal center B-cell differentiation. Immunological Reviews. 270: 8-19.

doi: 10.1186/s13578-016-0075-9 Marchwicka, A., Corcoran, A., Berkowska, K. and Marcinkowska, E. (2016) Restored expression of vitamin D receptor and sensitivity to 1,25‑dihydroxyvitamin D3 in response to disrupted fusion FOP2–FGFR1 gene in acute myeloid leukemia cells. Cell & Bioscience. 6:7.

Sugrue, T., Calvo-Asensio, I and Ceredig R. (2016) The radio-resistance of mesenchymal stromal cells and their potential role in the management of radiation injury. In “The Biology and Therapeutic Application of Mesenchymal Cells”. Ed: K Atkinson. Pub: Wiley Blackwell Publishers, Hoboken, New Jersey, USA. In press.

doi:10.1016/j.jsbmb.2016.03.013 Marchwicka, A., Cebrat, M., Łaszkiewicz, A., Śnieżewski, Ł., Brown, Marcinkowska, E. (2016) Regulation of vitamin D receptor expression by retinoic acid receptor alpha in acute myeloid leukemia cells. Journal of Steroid Biochemistry & Molecular Biology. 159, 121-130.

doi:10.3390/ijms17020091 Corcoran, A., Nadkarni, S., Yasuda K., Sakaki T., Brown G., Kutner, A. and Marcinkowska, E. (2016) Biological evaluation of double point modified analogues of 1,25-dihydroxyvitamin D2 as potential anti-leukemic agents. International Journal of Molecular Sciences. 17: 91.

doi:10.3390/ijms17010076 Piotrowska, A., Wierzbicka, J., Nadkarni, S., Brown, G., Kutner, A and Zmijewski, M. (2016) Antiproliferative activity of double point modified analogs of 1,25-dihydroxyvitamin D2 against human malignant melanoma cell lines. International Journal of Molecular Sciences. 17: 76.

doi:10.1016/j.jsbmb.2015.09.014 Nachliely, M., Sharony, E., Kutner, A. and Danilenko, M. (2016) Novel analogs of 1,25-dihydroxyvitamin D2 combined with a plant polyphenol as highly efficient inducers of differentiation in human acute myeloid leukemia cells. Journal of Steroid Biochemistry & Molecular Biology. 164: 59-65.

doi:10.1016/j.jsbmb.2015.08.022 Nadkarni, S., Chodynski, M., Krajewski, K., Cmoch, P., Marcinkowska, E., Brown, G. and Kutner, A. (2016) Convergent synthesis of double point modified analogs of 1α,25-dihydroxyvitamin D2 for biological evaluation. Journal of Steroid Biochemistry and Molecular Biology. 164: 45-49.

doi:10.1016/j.jsbmb.2015.09.033 Corcoran, A., Bermudez, M. A., Seoane, S., Perez-Fernandez, R., Krupa, M., Pietraszek, A., Chodyński, M., Kutner, A., Brown, G. and Marcinkowska, E. (2016) Biological evaluation of new vitamin D2 analogues. Journal of Steroid Biochemistry and Molecular Biology. 164: 66-71.

doi:10.1016/j.jsbmb.2015.08.025 Bolla, N. R., Corcoran, A., Yasuda, K., Chodyński, M., Krajewski, K., Cmoch, P., Marcinkowska, E., Brown, G., Sakaki, T. and Kutner, A. (2016) Synthesis and evaluation of geometric analogs of 1α,25-dihydroxyvitamin D2 as potential therapeutics. Journal of Steroid Biochemistry & Molecular Biology. 164: 50-55.

doi:10.1016/j.jsbmb.2015.10.015 Kozielewicz, P., Grafton, G., Kutner, A., Curnow S. J., Gordon, J. and Barnes, N. M. (2016) Novel vitamin D analogues; cytotoxic and anti-proliferative activity against a diffuse large B-cell lymphoma cell line and B-cells from healthy donors. Journal of Steroid Biochemistry & Molecular Biology. [Epub ahead of print].

doi:10.1089/scd.2014.0235 Wai, H.A., Kawakami, K., Wada, H., Muller, F., Vernallis, A. B., Brown, G. and Johnson, W. E. B. (2015) The development and growth of tissues derived from neural crest and primitive mesoderm is dependent on the ligation status of retinoic acid receptor gamma: evidence that retinoic receptor gamma functions to maintain stem/progenitors in the absence of retinoic acid. Stem Cells and Development. 24: 507-519 [Featured article].

doi:10.1186/s13287-015-0139-5 Baustian, C., Hanley, S. and Ceredig, R. (2015) Isolation, selection and culture methods to enhance clonogenicity of mouse bone marrow derived mesenchymal stromal cell precursors. Stem Cell Research & Therapy. 6: 151.

doi:10.3109/10408363.2015.1021412 Brown, G., Mooney, C. J., Alberti-Servera, L., Von Muenchow, L., Toellner, K-M., Ceredig, R. and Rolink, A. (2015) Versatility of stem and progenitor cells and the instructive actions of cytokines on haematopoiesis. Critical Reviews in Clinical Laboratory Sciences. 52: 168-179.

doi:10.18632/oncotarget.6145 Brown, G. and Sanchez-Garcia, I. (2015) Is lineage decision-making restricted during tumoral reprograming of haematopoietic stem cells? Oncotarget. 6: 43326-43341.

doi:10.1002/eji.201444681 Gehre, N., Nusser, A., Von Muenchow, L., Tussiwand, R., Engdahl, C., Capoferri, G., Bosco, N., Ceredig, R. and Rolink, A. G. (2015) A stromal cell-free culture system generates mouse pro-T cells that can reconstitute T-cell compartments in vivo. European Journal of Immunology. 45: 932-942.

doi:10.2174/1381612821666141205125113 Nadkarni, S., Chodyński, M., Corcoran, A., Marcinkowska, E., Brown, G. and Kutner, A. (2014) Double point modified analogs of vitamin D as potent activators of vitamin D receptor. Current Pharmaceutical Design. 21: 1241-1763.

doi: http://dx.doi.org/10.12968/joan.2014.3.6.276 Hughes, P. J., Kutner, A. and Brown, G. (2014) Understanding the physiology, pharmacology and therapeutic uses of vitamin D. Journal of Aesthetic Nursing. 3: 276-285.

doi:10.1016/j.imlet.2014.01.011 Von Muenchow, L., Engdahl, C., Karjalainen, K. and Rolink, A. G. (2014) The selection of mature B cells is critically dependent on the expression level of the co-receptor CD19. Immunology Letters. 160: 113-119.

doi:10.1517/13543776.2014.898061 Bolla, N. R., Marcinkowska, E., Brown, G. and Kutner, A. (2014) Retiferols – synthesis and biological activity of a conceptually novel class of vitamin D analogs. Expert Opinion on Therapeutic Patents. 24: 633-646.

doi: http://dx.doi.org/10.12968/npre.2013.11.7.344 Hughes, P. J., Kutner, A. and Brown, G., (2013) Shedding light on the physiology and pharmacology of vitamin D. Nurse Prescribing. 11: 344-352.

Books

Diversity, Versatility, and Leukaemia. G Brown and I Sanchez-Garcia (2016). Nova Science Publishers Inc., New York. ISBN: 978-1-63484-781-0

Special Journal Issue

The Biology and Treatment of Acute Myeloid Leukaemias. (A special issue of International Journal of Molecular Sciences). Eds: G Brown and E Marcinkowska, June 2017. MDPI, Basel, Switzerland.

http://www.mdpi.com/journal/ijms/special_issues/biology_treatment_myeloid_leukaemias

Posters

Camarillo et al., Single dose stromal cell transplants ameliorate cytopenia caused by depletion of FAPα-expressing cells, 1-4 February 2017, Haematopoiesis Keystone Symposium, Alberta, Canada

Camarillo et al., FAP+ stromal cells are necessary and sufficient to maintain marrow homeostasis, 6 - 9 Dec 2016, British Society for Immunology, Liverpool, England

Corcoran et al., Structure-function analysis of the analogues of 1,25-dihydroxyvitamin D3 as potential candidates for cell differentiation therapy for AML, 31 August - 3 September 2016, EMBO Chemical Biology Conference, Heidelberg, Germany

Urban et al., 2d, a dual polyamine analogue and LSD1 inhibitor, enhances differentiation-inducing potential of retinoic acid in neuroblastoma, 16 - 17 June 2016, ICR Conference, Royal Holloway, Egham, England.

Calvo Asensio et al., Effects of hypoxia on the radio-resistance of mouse thymic epithelial cells. 5 - 9 June 2016, ThymUS Conference, Maui, Hawaii.

Garcia Ibanez et al., The CCL19_CCL21 axis regulates Memory B cell appearance and migration, 20 April 16, Festival of Graduate Research, Medical School, University of Birmingham, England.

García Ibáñez et al., The CCL19/CCL21 axis regulates memory B cell appearance and migration, 28 February - 1 March 2016, Keystone Symposium ‘T follicular helper cells and germinal centres’, Monteray, USA.

Ribeiro et al., Qualitative effects of hypoxia on the composition of extracellular matrix produced by a cloned mouse mesenchymal stromal cell, 2 – 4 December 2015, 2nd Annual MBI meeting, University College Dublin, Dublin, Ireland.

Urban et al., Development of differentiation assay in neuroblastoma to elucidate the role of MYCN in differentiation, 26 – 27 November 2015, Newcastle, England.

Bolla et al., Design and synthesis of retiferols as a new class of des - CD vitamin D analogs, 16 - 17 October 2015, International conference, Vitamin D – Minimum, Maximum, Optimum, Warsaw, Poland.

Von Muenchow et al., Interplay between Flt3L and IL-7 in early haematopoietic development, 28 - 30 May 2015, PhD Retreat, Wolfsberg, Germany.

Camarillo et al., Life without MSCs: determining the effects of stromal cell loss, 27 May 2015, National University of Ireland, Galway, Ireland.

Alberti-Servera et al., Transcriptome analysis of EPLM subpopulations with various developmental potentials, 25 May 2015, Scientific DBM PhD Retreat, Schwarzsee, Switzerland.

García Ibáñez et al., ACKR4 deficiency causes germinal centres with deregulated shape, 16 - 21 May 2015, EMBO-ESF Symposium, San Feliu de Guixois, Spain

Calvo Asensio et al., Effects of hypoxia on the radio-resistance of mouse mesenchymal stromal cells and thymic epithelial cells, 12 - 17 May 2015, Keystone Symposium on Molecular and Cellular Biology, Dublin, Ireland.

Bolla et al., Synthesis and biological activity of analogs of 1,25-dihydroxyvitamin D2, 21 - 24 April 2015, 18th Workshop on Vitamin D, Delft, the Netherlands.

Corcoran et al., Biological evaluation of new vitamin D2 analogues, 21 - 24 April 2015, 18th Workshop on Vitamin D, Delft, the Netherlands.

Kozielewicz et al., Calcitriol and side-chain modified vitamin d analogues: their impact on healthy and malignant B cells, 21 - 24 April 2015, 18th Workshop on Vitamin D, Delft, the Netherlands.

Nadkarni et al., Design, synthesis, characterization and biological activity of vitamin D analogs, 21 - 24 April 2015, 18th Workshop on Vitamin D, Delft, the Netherlands.

Von Muenchow et al., Interplay between Flt3L and IL-7 in early haematopoietic development, 8 - 13 March 2015, DGfI Spring School, Germany.

Alberti-Servera et al., Transcriptome analysis of EPLM subpopulations with various developmental potentials, 9 March 2015, Spring School on Immunology, Ettal, Bavaria, Germany.

Camarillo et al., Life without MSC? 3 - 5 December 2014, Berlin-Brandenburg School for Regenerative Therapies Symposium, Berlin, Germany.

García Ibáñez et al., Atypical chemokine receptor 4, ACKR4, in B cell activation, 1-4 December 2014, British Society for Immunology Congress, Brighton, England.

Ribeiro et al., Effects of hypoxia on the extracellular matrix produced by the mouse MS-5 mesenchymal stromal cell line, 19 - 21 November 2014, Matrix Biology, Ireland.

Corcoran et al., Biological evaluation of new vitamin D2 analogues, 9 – 12 September 2014, BIO 2014, University of Warsaw, Poland.

García Ibáñez et al., Chemokine-based decisions in germinal centre, 10 June 2014, Research Poster Conference, University of Birmingham, England

Sugrue, Calvo Asensio et al., Effects of hypoxia on the radio resistance of mouse mesenchymal stromal cells and thymic epithelial cells, 28 May 2014, Postgraduate Research Poster Day, Dublin, Ireland.

Sugrue, Calvo Asensio et al., Hypoxia enhances the radio resistance of mouse mesenchymal stromal cells, 25 - 26 February 2014, Irish Cytometry Society Meeting, Dublin, Ireland.

Ribeiro et al., B lymphopoiesis in pregnant women, 25 - 26 February 2014, Irish Cytometry Society Meeting, Dublin, Ireland.

McLoughlin, Ribeiro et al., Monocytes and T cells in haemochromatosis, 25 - 26 February 2014, Irish Cytometry Society Meeting, Dublin, Ireland.

Ribeiro et al., Development of a flow cytometry-based assay for the immunomodulatory properties of human mesenchymal stromal cells, 25 - 26 February 2014, Irish Cytometry Society Meeting, Dublin, Ireland.

Urban et al., Development of differentiation assay for neuroblastoma, 21 January 2015, ICR Science Afternoon, London, England.

Alberti-Servera et al., Lineage-affiliated specification events during early stages of haematopoietic development, 14 January 2014, DBM PhD student retreat, Hasliberg, Switzerland.