Stochastic Optical Reconstruction Microscopy (STORM) and Photoactivated Localization Microscopy (PALM) are based on recurrently determining the position of single fluorescence emitters in live or fixed samples. The repeated localizations of individual molecules is then used to reconstruct super-resolved images. Applications include studying the nanodomains organization of signalling complexes or small subcellular structures like mitochondria or the cytoskeleton.
Microscope Details
- Sample size: Single cells, tissue slices, organoids
- Main application: Stochastic Optical Reconstruction Microscopy (STORM)
- Modalities: dSTORM (2D and 3D), PALM, TIRF
- Spatial Resolution: ~20 nm lateral, ~50 nm axial
- Temporal resolution: medium (20 fps)
- Environmental control: Temperature, humidity and CO2
- Excitation Lasers: 405, 488, 560, 640 nm