SIM (Structured Illumination Microscopy) is a form of super resolution microscopy that allows living and fixed fluorescent samples to be observed at approximately twice the resolution of a standard fluorescence microscope. Imaging at this higher resolution can reveal structural sub-cellular organisation which would otherwise be masked on a standard microscope.
Microscope Details
- Sample size: single cells, organoids
- Main application: Structured Illumination Microscopy (SIM)
- Modalities: 2D and 3D SIM, TIRF SIM
- Spatial resolution: ~115 nm lateral, ~270 nm axial
- Temporal resolution: medium (~15 fps)
- Environmental control: Temperature, humidity and CO2
- Excitation Lasers: 405, 488, 560, 640 nm