Dr Tamara Miljus

Department of Metabolism and Systems Science
Postdoctoral Research Fellow

Contact details

Email: t.miljus@bham.ac.uk

Address: Office 329, IBR
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Tamara is a postdoctoral research fellow in the group of Professor Davide Calebiro. She is using fluorescence-based single-molecule microscopy to study dynamics of interactions between G protein-coupled receptors (GPCRs) and arrestins.

Qualifications

Doctor of Sciences, ETH Zurich, Switzerland, 2018
MSc in Chemistry, fields biochemistry and organic chemistry, University of Zagreb, Croatia, 2015
BSs in Chemistry, University of Zagreb, Croatia, 2013

Biography

Prior to joining IMSR, Tamara completed her PhD at the Paul Scherrer Institute in Switzerland, investigating molecular mechanisms of biased signalling in cannabinoid CB2 receptor. During PhD, she spent 8 months in Professor Michel Bouvier’s lab at the University of Montreal using BRET-based biosensors in live cells to assess signalling of cannabinoid receptors, including high-throughput ligand and mutant screens.

Publications

Tippett DN, Hoare B, Miljus T, Sykes DA, Veprintsev DB. ThermoFRET: A novel nanoscale G protein coupled receptor thermostability assay functional in crude solubilised membrane preparations. BioRxiv Preprint (2020)

Miljus T, Sykes DA, Harwood CR, Vuckovic Z, Veprintev DB. GPCR Solubilization and Quality Control. Methods Mol Biol 2127 (2020) 105-127

Heydenreich FM*, Miljuš T*, Milić D, Veprintsev DB. High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach. Bio-protocol 10 (2020) e3484

Sarott R, Westphal M, Pfaff P, Korn C, Sykes D, Thais G et al. Highly Specific, Fluorescent Cannabinoid Type 2 Receptor Probes Enable Applications in Microscopy, Flow Cytometry and FRET-based Binding Assays. ChemRxiv Preprint (2019)

Thais G, Brennecke B, Atz K, Korn C, Sykes D, Sarott R et al. Drug Derived Fluorescent Probes for the Specific Visualization of Cannabinoid Type 2 Receptor - A Toolbox Approach. ChemRxiv Preprint (2019)

Heydenreich FM*, Miljus T*, Benoit R, Jaussi R, Milic D, Veprintsev DB. High-throughput mutagenesis using a two-fragment PCR approach. Scientific Reports 7 (2017) 6787

Venkatakrishnan AJ, Deupi X, Lebon G, Heydenreich FM, Flock T, Miljus T, Balaji S, Bouvier M, Veprintsev DB, Tate CG, Schertler GFX, Babu MM. Diverse activation pathways in class A GPCRs converge near the G protein-coupling region. Nature 536 (2016) 484-487

View all publications in research portal