BMG CLARIOstar Plus Plate Reader

The CLARIOstar Plus is a multi-mode microplate reader with advanced LVF Monochromators™, highly sensitive filters, and an ultra-fast UV/vis spectrometer. The Enhanced Dynamic Range and automatic focus technologies make manual settings superfluous and detection optimisation easier than ever. Combined with atmospheric control, temperature incubation, multiple shaking, well scanning and reagent injection, our CLARIOstar Plus is the ideal platform for any live cell-based assay.

Features

  • Innovative monochromators for increased sensitivity

  • Fully-flexible wavelengths and bandwidths

  • Spectral scanning in absorbance, fluorescence intensity, and luminescence

  • Largest dynamic range with no manual intervention

  • Rapid full-plate autofocus

  • Dedicated features for live cell-based assays 

  • Ultra-fast data sampling (100 measurements/second)

  • Easily integrated into automated systems

Technical Specifications


 

 Detection Modes

Fluorescence intensity (incl. FRET)

Fluorescence polarization

Luminescence (flash and glow) - incl. BRET

Time-resolved fluorescence

TR-FRET

UV/vis absorbance spectra

 Measurement Modes

Top and bottom reading

Endpoint and kinetic

Sequential multi-excitation

Sequential multi-emission

Spectral scanning (fluorescence, luminescence, absorbance)

Ratiometric measurements

Well scanning 

 Microplate Formats

 6- to 1536-well plates, user-definable

 Light Sources

High energy xenon flash lamp

Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlex™

 Detectors

Low-noise photomultiplier tube

Red-shifted photomultiplier tube

CCD spectrometer

 Wavelength Selection

Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators

Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well

 Spectral Range

Filters
EXTR LPTR 665-10 and 620-10 set.

LVF Monochromators™
FI: 320 - 740 nm
LUM: 320 - 740 nm

Linear Variable Dichroic
340 - 740 nm

Spectrometer
ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm

 Sensitivity

FI monochromator - (top) - < 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL).

FI monochromator - (bottom) - < 3.0 pM (< 150 amol/well FITC, 384g, 50 μL).
Dynamic Range: 8 decades in a single measurement.

FP - < 0.5 mP SD at 1 nM FITC (384sv, 20 μL).

HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL).
Delta F > 880% (High Calibrator).
Delta F > 30% (Low Calibrator).

TRF - < 20 fM europium, 384, 80 μL.

LUM - < 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL).
Dynamic range: 8 decades in a single measurement.

ABS with spectrometer.
Selectable spectral resolution: 1, 2, 5, and 10 nm.
OD range: 0 to 4 OD; photometric resolution: 0.001 OD.
Accuracy: < 1% at 2 OD.
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD.
Linearity: ≤ 0.8% at 2.0 OD.

 Read Times

Flying mode (1 flash) - 8 s (96), 15 s (384), 28 s (1536)

10 flashes - 19 s (96), 57 s (384), 3 min 4 s (1536

 Shaking

Linear, orbital, and double-orbital with user-definable time and speed

 Incubation

 +3 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

 Software

Multi-user Reader Control and MARS Data Analysis Software included

FDA 21 CFR Part 11 compliant

Integrated fluorophore library

 Z-Adjustment

 Automatic focal height adjustment (0.1 mm resolution)

 Reagent Injection

Up to 2 built-in reagent injectors

Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)

Variable injection speed up to 420 µL/s

Reagent back flushing

 THERMOstar

 Microplate incubator and shaker

Atmospheric Control Unit (ACU)

 Actively regulates O2 and CO2 - 0.1-20%

Accessories 

Atmospheric Control UnitACU jpeg

The Atmospheric Control Unit (ACU) for the CLARIOstar Plus, is the ideal solution for live cell-based applications, from standard cell growth to hypoxic assays or pH control. The ACU provides a complete solution to fully and independently regulate both O2 and CO2 gas levels within the microplate reader chamber. From standard proliferation to hypoxic or cytotoxicity assays, the ACU provides the optimal environment for any live cell assay. In combination with temperature control, shaking options, and bottom reading detection, the ACU offers a versatile walk-away solution for long-term cell-based assays. 

Features

  • O2 control range: 0.1 - 20%

  • CO2 control range: 0.1 - 20%

  • Gas ramping function - The CLARIOstar Plus with ACU offers the capability to run O2 and CO2 gas ramps. For instance, the ACU can deprive O2 and then rapidly re-oxygenate back to physiological conditions, keeping meanwhile steady CO2 levels. This capability enhances live cell-based assays and supports ischemia-reperfusion research, as disease models such as ischemia-reperfusion can be reproduced in vitro in a microplate reader.

  • Gas value trackability in MARS Data Analysis Software 

  • LCD touchscreen with intuitive user interface and gas concentration curve display

  • Up to 10 user-definable presets

  • Superior valve control for minimal gas consumption

  • Integrated gas pressure regulators and sensors with acoustic alarm

  • Altitude correction for accurate gas regulation

Applications

  • Proliferation assays and cell viability

  • Migration and invasion studies

  • Hypoxia

  • Ischemia/reperfusion

  • Angiogenesis

  • Cell Cytotoxicity studies

  • Viral uptake

Off-the-shelf Assays

Metabolite Detection Assays  

Metabolite detection assay kits

Cellular Health and Viability Assays 

Cellular Health and Viability Assays

NAD(P)/NAD(P)H Assays

NAD(P)H Assay Kits

Further Information   

https://www.bmglabtech.com/en/clariostar-plus/

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