Dr Jonathan Barlow PhD

Dr Jonathan Barlow

School of Sport, Exercise and Rehabilitation Sciences
Scientific Research Director, Cellular Health and Metabolism Facility

Contact details

Email
j.p.barlow@bham.ac.uk
All enquiries for the CHMF:
mpcentre@contacts.bham.ac.uk
Twitter
@Barlow_JP
Address
Lab 210
Cellular Health and Metabolism Facility
School of Sport, Exercise and Rehabilitation Sciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Dr. Barlow has more than 10 years’ experience using extracellular flux analysis and high resolution respirometry for assessing ‘real-time’ cellular metabolism in cells and tissues. His expertise covers in-depth knowledge of cellular bioenergetics with specific skills using Seahorse Extracellular Flux (XF) Analysers and Oroboros O2K high-resolution FluoRespirometry. He also has experience exploring cellular metabolism in cells and tissues using multimode optical plate readers and has expertise in metabolite detection assays. His own research interests are targeted at understanding the involvement of mitochondria in human pathologies linked to diabetes, cancer, autoimmune disease, and neurodegeneration.

Dr. Barlow provides comprehensive training programmes in techniques used to explore ‘real-time’ cellular metabolism. And offers academic consultancy to companies and institutions that require bespoke metabolic assay development using Seahorse XF technology or need assistance with analysing and interpreting Seahorse XF data.  


Publications

Coltman, N. J., Rochford, G., Hodges, N. J., Ali-Boucetta, H., Barlow, J. P. Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids using Extracellular Flux Analysis. J. Vis. Exp. (180), e63346, doi:10.3791/63346 (2022) 

Daniel G. Sadler, Jonathan Barlow, Richard Draijer, Helen Jones, Dick H. J. Thijssen, Claire E. Stewart, "(–)-Epicatechin Alters Reactive Oxygen and Nitrogen Species Production Independent of Mitochondrial Respiration in Human Vascular Endothelial Cells", Oxidative Medicine and Cellular Longevity, vol. 2022, Article ID 4413191, 13pages, 2022. https://doi.org/10.1155/2022/4413191 

Prolyl-4-hydroxylase 3 maintains β cell glucose metabolism during fatty acid excess in mice. (2021). Prolyl-4-hydroxylase 3 maintains β cell glucose metabolism during fatty acid excess in mice. 6 (16). http://doi.org/10.1172/jci.insight.140288

Jonathan P. Barlow, Kristian Karstoft, Andreas Vigelsø, Martin Gram, Jørn W. Helge, Flemming Dela, Kirk Pappan, Donna O’Neil, Warwick Dunn, Thomas P.J. Solomon,Beta-aminoisobutyric acid is released by contracting human skeletal muscle and lowers insulin release from INS-1 832/3 cells by mediating mitochondrial energy metabolism,Metabolism Open,Volume 7, 2020,100053,ISSN 2589-9368, https://doi.org/10.1016/j.metop.2020.100053

Seabright, A. P., Fine, N. H. F., Barlow, J. P., Lord, S. O., Musa, I., Gray, A., et al. (2020). AMPK activation induces mitophagy and promotes mitochondrial fission while activating TBK1 in a PINK1-Parkin independent manner. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 34(5), 6284–6301. http://doi.org/10.1096/fj.201903051R

Barlow, J. and Solomon, T. (2018). Conditioned media from contracting skeletal muscle potentiates insulin secretion and enhances mitochondrial energy metabolism of pancreatic beta-cells. Metabolism.  Impact factor: 5.963. PMID: 30445139

Barlow, J., Carter, S., and Solomon, T. (2018). Skeletal muscle to pancreatic beta-cell crosstalk: Probing the effect of IL-6 on insulin secretion by INS-1 832/3 insulinoma cells under diabetic-like conditions. International journal of Molecular Sciences. Impact Factor: 3.226. PMID: 29966345

Barlow, J., Solomon, T., and Affourtit, C. (2018). Pro-inflammatory cytokines attenuate glucose-stimulated insulin secretion from INS-1E insulinoma cells by restricting mitochondrial pyruvate oxidation capacity – novel mechanistic insight from real-time analysis of oxidative phosphorylation. PLOS one. Impact factor: 2.806. PMID: 29953508

Affourtit, C., Alberts, B., Barlow, J., Carré, J. and Wynne, A. (2018). Control of pancreatic β-cell bioenergetics. Biochemical Society Transactions. Impact factor: 2.765. PMID: 29666215

Barlow, J. and Solomon, T. (2017). Do skeletal muscle-secreted factors influence the function of pancreatic beta cells? AJP Endocrinology and Metabolism. Impact factor: 4.142. PMID: 29208613

Barlow J, Jensen VH, Jastroch M, and Affourtit C. (2016) Palmitate-induced impairment of glucose-stimulated insulin secretion precedes mitochondrial dysfunction in mouse pancreatic islets. Biochemical Journal. 4, 487–96. Impact factor: 3.797. PMID: 26621874

Barlow, J., Hirschburg, V. and Affourtit, C. (2014) Uncoupling protein-2 attenuates palmitoleate protection against the cytotoxic production of mitochondrial reactive oxygen species in INS-1E insulinoma cells. Redox Biology. 4, 14-22. Impact Factor: 6.337. PMID: 25482405

Barlow, J. and Affourtit, C. (2013) Novel insights in pancreatic beta cell glucolipotoxicity from real-time functional analysis of mitochondrial energy metabolism in INS-1E insulinoma cells. Biochemical Journal. 456, 417-426. Impact Factor: 3.797. PMID: 24099598

Barlow, J., Hirschberg, V., Brand, M. D. and Affourtit, C. (2013) Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells. Methods of Enzymology. 528, 257–267. Impact Factor: 1.972. PMID: 23849870

Barlow, J., Hirschberg, V. and Affourtit, C. (2013). On the role of mitochondria in pancreatic beta cells. In: Research on Diabetes 1 (ISBN: 978-1-922227-21-8), edited by iConcept Press, printed in Hong Kong.