Selected Publications
A more comprehensive list is available on Google Scholar
- Cao, P. Müller, T.K.H., Ketterer, B., Ewert, S., Theodosiou, E., Thomas, O.R.T. and Franzreb, M. (2015) Integrated system for temperature-controlled fast protein liquid chromatography. II. Optimized adsorbents and ‘single column continuous operation’ J. Chromatogr. A, online 3 June 2015, doi: 10.1016/jchroma.2015.05.039.
- Hsu, C.-C., Thomas, O.R.T. and Overton, T.W. (2015) Periplasmic expression in and release of Fab fragments from Escherichia coli using stress minimization. J. Chem. Technol. Biotechnol., online 21 March 2015, doi: 10.1002/jctb.4672.
- Jamshad, M., Grimard, V., Idini, I., Knowles, T.J., Dowle, M.R., Schofield, N. Sridhar, P., Lin, Y-P., Finka, R., Wheatley, M., Thomas, O.R.T., Palmer, R.E., Overduin, M., Govaerts, C., Ruysschaert, J-M., Edler, K.J., Dafforn, T.R. (2015) Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. Nano Research 8(3):774-789; doi: 10.1007/s12274-014-0560-6.
- Fischer, I., Gärtner, Hsu, C-C., Müller, C., Overton, T.W., Thomas, O.R.T., Franzreb, M. (2013) ‘Continuous protein purification using functionalized magnetic nanoparticles and aqueous micellar two-phase systems’, J. Chromatogr. A, 1305: 7-16. DOI: 10.1016/j.chroma.2013.06.011
- Olszewski, P., Willett, T.C., Theodosiou, E., Thomas, O.R.T., Walsh, J. L. (2013) ‘In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas’, Appl. Phys. Lett., 102: 202104-1 – 20104-5. DOI: 10.1063/1.4807931.
- Müller, T.K.H., Cao, P., Ewert, S., Wohlgemuth, J., Liu, H., Willett, T.C., Theodosiou, E., Thomas, O.R.T., Franzreb, M. ‘Integrated system for temperature-controlled FPLC comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor (TCZR) arrangement’, J. Chromatogr. A, 1285: 97 – 109. DOI: 10.1016/j.chroma.2013.02.025.
- Brown, G.N., Müller, C., Theodosiou, E., Franzreb, M., Thomas, O.R.T. (2013) ‘Multi-cycle Recovery of Lactoferrin and Lactoperoxidase from Crude Whey using Fimbriated High-Capacity Magnetic Cation Exchangers and a Novel ‘Rotor-Stator’ High-Gradient Magnetic Separator’, Biotechnology and Bioengineering, 110(6): 1714 –1725. DOI: 10.1002/bit.24842.
- Jamshad, M., Lin, Y-P., Knowles, T.J. Parslow, R.A. Harris, C., Wheatley, M., Poyner, D.R., Bill, R.M., Thomas, O.R.T., Overduin, M. and Dafforn, T.R. (2011) ‘Surfactant-free purification of membrane proteins with intact native membrane environment’, Biochem. Soc. Trans., 39(3): 813-818. DOI: 10.1042/BST0390813.
- Arpanaei, A., Jensen, B.W., Theodosiou, E. Kingshott, P., Hobley, T.J., Thomas, O.R.T. (2010) ‘Surface modification of chromatography adsorbents by low temperature low pressure glow discharge plasma’, J. Chromatogr. A, 1217(44): 6905-6916. DOI: 10.1016/j.chroma.2010.08.069.
- Becker, J.S, Thomas, O.R.T. and Franzreb, M. (2009) ‘Protein separation with magnetic adsorbents in micellar aqueous two-phase systems’, Sep. Sci. Technol., 65(1): 46-53.
- Arpanaei, A., Heebøll-Nielsen, A., Hubbuch, J.J., Thomas, O.R.T. and Hobley, T.J. (2008) ‘Critical evaluation and comparison of fluid distribution systems for industrial scale expanded bed adsorption chromatography column’, J. Chromatogr. A, 1198-1199: 131-139. DOI: 10.1016/j.chroma.2008.05.44.
- Faber, C., Hobley, T.J.,Mollerup, J., Thomas, O.R.T. and Kaasgaard, S.G. (2008) ‘Factors affecting the solubility of Bacillus halmapalus a-amylase’, Chem. Eng. Processing, 47: 1007-117. DOI: 10.1016/j.cep.2007.02.015.
- Faber C., Hobley, T.J., Mollerup, J., Thomas, O.R.T. and Kaasgaard, S.G. (2007) ‘Study of the solubility of a modified Bacillus licheniformis a-amylase around the isoelectric point’, J. Chem. Eng. Data, 52: 707-713.
- Ebner, N., Gomes, C.S., Hobley, T.J., Thomas, O.R.T. and Franzreb, M. (2007) ‘Filter capacity predictions for the capture of superparamagnetic microparticles by high-gradient magnetic separation (HGMS)’ IEEE Trans. Magnetics, 43(5): 1941-1949.
- Bak, H., Kyhse-Andersen, J. and Thomas, O.R.T. (2007) ‘High-throughput immunoturbidimetric assays for in-process determination of polyclonal antibody concentration and functionality in crude samples’, J. Chromatogr. B, 848, 142-150.
- Bak, H, Thomas, O.R.T. and Abildskov, J. (2007)‘Lumped parameter model to predict initial breakthrough profiles from preparative chromatographic capture of polyclonal rabbit antibodies from clarified antiserum’, J. Chromatogr. B, 848, 131-141.
- Bak, H. and Thomas, O.R.T. (2007) ‘Evaluation of commercial chromatographic adsorbents for the direct capture of polyclonal rabbit antibodies from clarified antiserum’, J. Chromatogr. B, 848, 116-130.
- Franzreb, M., Siemann-Herzberg, M., Hobley, T.J., and Thomas, O.R.T. (2006) ‘Product recovery by high-gradient magnetic fishing (HGMF).’ In Process Scale Bioseparations for the Biopharmaceutical Industry, eds. A.A. Shukla, M.R. Etzel, S. Gadam, Taylor and Francis – CRC press (ISBN: 1574445170, publication date: 12/07/2006).
- Franzreb, M., Siemann-Herzberg, M., Hobley, T.J. and Thomas, O.R.T. (2006) ‘Protein purification using magnetic adsorbent particles’, Appl. Microb. Biotechnol., 70: 505-516.
- Guillaumie, F., Justesen, S.F.L., Mutenda, K.E., Roepstorff, P., Jensen, K.J. and Thomas, O.R.T. (2006) ‘Fractionation, solid-phase immobilization and chemical degradation of long pectin oligogalacturonides. Initial steps towards sequencing of oligosaccharides,’ Carbohydr. Res., 341(1): 118-129.
- Ferré, H., Ruffet, E., Nielsen, L-L.B, Holst Nissen, M., Hobley, T.J., Thomas, O.R.T. and Buus, S. (2005) ‘A novel system for continuous protein refolding and on-line capture by expanded bed adsorption,’ Protein Science, 14: 2141-2153.
- Meyer, A., Hansen, D.B., Gomes, C.S.G., Hobley, T.J., Thomas, O.R.T. and Franzreb, M. (2005) ‘Demonstration of a strategy for product purification by high-gradient magnetic fishing: Recovery of superoxide dismutase from unconditioned whey,’ Biotechnol. Progr., 21, 244-254.
- Regenberg, B., Krühne, U., Beyer, M., Pedersen, L.H, Simón, M., Thomas, O.R.T., Nielsen, J. and Ahl, T. (2004) ‘Use of laminar flow patterning for miniaturised biochemical assays,’ Lab-on-a-Chip, 4: 654-657.
- Heebøll-Nielsen, A., Justesen, S.F.L. and Thomas, O.R.T. (2004) ‘Fractionation of whey proteins with high-capacity superparamagnetic ion-exchangers,’ J. Biotechnol., 113: 247-262.
- Heebøll-Nielsen, A., Justesen, S.F.L. Hobley, T.J. and Thomas, O.R.T. (2004) ‘Superparamagnetic cation-exchange adsorbents for bioproduct recovery from crude process liquors by high-gradient magnetic fishing,’ Sep. Sci. Technol., 39(12): 2891-2914.
- Heebøll-Nielsen, A., Dalkiær, M., Hubbuch, J.J. and Thomas, O.R.T. (2004) ‘Superparamagnetic adsorbents for high-gradient magnetic fishing of lectins out of legume extracts.’ Biotechnol. Bioeng., 87(3): 311-323
View all publications in research portal