Professor Tim Dafforn PhD

Dr Tim Dafforn

School of Biosciences
Professor of Biotechnology
Knowledge Exchange lead for Biosciences

Contact details

Address
418, School of Biosciences
University of Birmingham
Edgbaston
Birmingham
B15 2TT
UK

Professor Tim Dafforn has established himself as an expert in biophysical spectroscopy with a keen interest in synthetic biology. Professor Dafforn has developed a number of projects from inception through to commercial realization.

Qualifications

BSc (Hons) Biochemistry(Cardiff University)

PhD ProteinEngineering (Bristol University) 

Biography

HARNESSING ENGINEERING BIOLOGY AND BIONANOTECHNOLOGY 

Professor Dafforn's fundamental research interests focus on the application of nano-technology approaches to enhance the exploitation of biomolecules for public good. His research has led to the development of new methods (SMALPs) to extract and purify membrane proteins and the development of novel diagnostics systems. One of the most high profile of these systems has been the development of RTF-EXPAR, the worlds fastest method for detecting pathogen RNA (including SARS-CoV-2).  

BUSINESS AND POLICY IMPACT 

Professor Dafforn is also keenly interested in methods to maximise the impact of research. He has worked at the highest levels of UK government as Chief Scientific Advisor to the Department of Business Energy and Industrial Strategy developing policy on science exploitation and entrepreneurship education. He has also founded two businesses to exploit research produced within his laboratory.

Teaching

Professor Dafforn enjoys teaching, and is keen on the adoption of modern teaching practices. His current teaching activities focus on delivering applied courses that enhance the ability of students to translate their science. 

Research

What are SMALPs, and how do they work?

Over the past 2 billion years life has been evolving to produce the wonderful biological diversity we see today. At the molecular level evolution has produced nano-molecular machines of wonderful complexity. Tim’s research is focused upon studying these wonders, understanding how they assemble and harnessing their function for good. Currently his studies have three streams of research:

  1. Studying and understanding the complex molecular machinery that underlies bacterial cell division.
  2. Developing novel nanoencapsulation methods that allow large scale production of membrane proteins.
  3. Building nano-assemblies that can be used in detection systems

The last two of these projects have provided seven patents that are currently the focus of two commercialisation projects. 

Other activities

While Science and Science Policy are Tim's work passions he believes that it is important to let your mind explore other areas in your leisure. He spends leisure time supporting Leicester Tigers Rugby team and obsessively watching F1. He also competes in long distant car rallies with a variety of 50 year old vehicles.

Publications

Recent publications

Article

Bruque, M, Alison, R, Vrønning Hoffmann, S, Jones, N, Aucamp, J, Dafforn, T & Thomas, O 2024, 'Analysis of the Structure of 14 Therapeutic Antibodies Using Circular Dichroism Spectroscopy', Analytical Chemistry, vol. 96, no. 38, pp. 15151-15159. https://doi.org/10.1021/acs.analchem.4c01882

Wang, X, Slater, A, Lee, SC, Harrison, N, Pollock, NL, Bakker, SE, Navarro, S, Nieswandt, B, Dafforn, TR, García, Á, Watson, SP & Tomlinson, MG 2024, 'Purification and characterisation of the platelet-activating GPVI/FcRγ complex in SMALPs', Archives of Biochemistry and Biophysics, vol. 754, 109944. https://doi.org/10.1016/j.abb.2024.109944

Li, HZ, Pike, ACW, Lotsaris, I, Chi, G, Hansen, JS, Lee, SC, Rödström, KEJ, Bushell, SR, Speedman, D, Evans, A, Wang, D, He, D, Shrestha, L, Nasrallah, C, Burgess-Brown, NA, Vandenberg, RJ, Dafforn, TR, Carpenter, EP & Sauer, DB 2024, 'Structure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2', Nature Communications, vol. 15, no. 1, 5503. https://doi.org/10.1038/s41467-024-48921-x

Cioccolo, S, Barritt, JD, Pollock, N, Hall, Z, Babuta, J, Sridhar, P, Just, A, Morgner, N, Dafforn, T, Gould, I & Byrne, B 2024, 'The mycobacterium lipid transporter MmpL3 is dimeric in detergent solution, SMALPs and reconstituted nanodiscs', RSC Chemical Biology. https://doi.org/10.1039/d4cb00110a

Harrison, N, Richardson, L, Pallini, C, Morano, I, Jinks, E, Cowley, J, Chan, H, Hill, HJ, Tuekprakhon, A, Li, Z, Matas de las Heras, C, Teodosio, A, Lavado, AS, Moring, R, Ashraf, A, Dafforn, TR, Grammatopoulos, DK, Gordon, J, Brady, CA, Young, LS, Barnes, NM, Stamataki, Z & Qureshi, OS 2023, 'A cell-based, SARS-CoV-2 spike protein interaction assay to inform the neutralising capacity of recombinant and patient sera antibodies', Frontiers in Virology, vol. 3, 1163385. https://doi.org/10.3389/fviro.2023.1163385

Little, HA, Ali, A, Carter, JG, Hicks, MR, Dafforn, TR & Tucker, JHR 2023, 'A plug-and-play aptamer diagnostic platform based on linear dichroism spectroscopy', Frontiers in Chemistry, vol. 11, 1040873. https://doi.org/10.3389/fchem.2023.1040873

Martin, A, Jemmett, P, Howitt, T, Wood, M, Burley, A, Cox, L, Dafforn, T, Welbourn, R, Campana, M, Skoda, MWA, Thompson, J, Hussain, H, Rawle, JL, Carla, F, Nicklin, CL, Arnold, T & Horswell, SL 2023, 'Effect of anionic lipids on mammalian plasma cell membrane properties', Langmuir, vol. 39, no. 7, pp. 2676-2691. https://doi.org/10.1021/acs.langmuir.2c03161

Pollock, NL, Lloyd, J, Montinaro, C, Rai, M & Dafforn, TR 2022, 'Conformational trapping of an ABC transporter in polymer lipid nanoparticles', The Biochemical journal, vol. 479, no. 2, pp. 145-159. https://doi.org/10.1042/BCJ20210312

Jemmett, PN, Milan, DC, Nichols, RJ, Howitt, T, Martin, A, Arnold, T, Rawle, JL, Nicklin, CL, Dafforn, TR, Cox, LR & Horswell, SL 2022, 'Influence of the lipid backbone on electrochemical phase behavior', Langmuir, vol. 38, no. 46, pp. 14290-14301. https://doi.org/10.1021/acs.langmuir.2c02370

Grime, R, Logan, R, Nestorow, S, Sridhar, P, Edwards, PC, Tate, CG, Klumperman, B, Dafforn, T, Poyner, DR, Reeves, PJ & Wheatley, M 2021, 'Differences in SMA-like polymer architecture dictate the conformational changes exhibited by the membrane protein rhodopsin encapsulated in lipid nano-particles', Nanoscale, vol. 13, no. 31, pp. 13519-13528. https://doi.org/10.1039/d1nr02419a

Vafaei, S, Allabush, F, Tabaei, SR, Male, L, Dafforn, TR, Tucker, JHR & Mendes, PM 2021, 'Förster resonance energy transfer nanoplatform based on recognition-induced fusion/fission of DNA mixed micelles for nucleic acid sensing', ACS Nano, vol. 15, no. 5, pp. 8517-8524. https://doi.org/10.1021/acsnano.1c00156

Carter, JG, Pfukwa, R, Riley, L, Tucker, JHR, Rodger, A, Dafforn, TR & Klumperman, B 2021, 'Linear dichroism activity of chiral poly(p-aryltriazole) foldamers', ACS Omega, vol. 6, no. 48, pp. 33231-33237. https://doi.org/10.1021/acsomega.1c06139

Hawkins, OP, Jahromi, CPT, Gulamhussein, AA, Nestorow, S, Bahra, T, Shelton, C, Owusu-mensah, QK, Mohiddin, N, O'rourke, H, Ajmal, M, Byrnes, K, Khan, M, Nahar, NN, Lim, A, Harris, C, Healy, H, Hasan, SW, Ahmed, A, Evans, L, Vaitsopoulou, A, Akram, A, Williams, C, Binding, J, Thandi, RK, Joby, A, Guest, A, Tariq, MZ, Rasool, F, Cavanagh, L, Kang, S, Asparuhov, B, Jestin, A, Dafforn, TR, Simms, J, Bill, RM, Goddard, AD & Rothnie, AJ 2021, 'Membrane protein extraction and purification using partially-esterified SMA polymers', Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1863, no. 12, 183758. https://doi.org/10.1016/j.bbamem.2021.183758

Patent

Tucker, J, Dafforn, T, Carter, J, Duprey, J-L, Orueta Iturbe, L & Hicks, MR Jun. 09 2022, Target RNA Detection, Patent No. WO2022117816A1.

Preprint

Harrison, N, Richardson, L, Pallini, C, Morano, I, Jinks, E, Cowley, J, Chan, H, Hill, HJ, Matas de las Heras, C, Teodosio, A, Lavado, AS, Dafforn, TR, Grammatopoulos, DK, Gordon, J, Brady, CA, Young, LS, Barnes, NM, Stamataki, Z & Qureshi, OS 2022 'A cell-based, spike protein binding assay highlights differences in antibody neutralising capacity for SARS-CoV-2 variants' bioRxiv. https://doi.org/10.1101/2022.06.24.496409

View all publications in research portal

Expertise

Professor Dafforn has extensive experience in national and international policy making in both science and Entrepreneurship policy. He has served as: 

a) Chief Scientific and Chief Entrepreneurial Advisor to the Department of Business Energy and Industrial Strategy.

And is currently:

a) Chair of the Institute of Engineering Technology's Innovation and Skills Panel

b) Member of the Ministerial led UK Engineering Biology Leadership Council

c) Member of the Defence Science Expert Committee at the Ministry of Defence